Sapient’s targeted protein assay services leverage targeted mass spectrometry to enable precise, quantitative measurement of predefined proteins of interest across a range of biological samples.
Our off-the-shelf assays encompass >12K proteins which can undergo further qualification
Amenable to plasma, serum, tissue, tumors, CSF, urine, saliva, cells, media, and more
Enables measure of specific protein isoforms, variants, and PTMs
Through these protein assay services, we deliver robust data for precise assessment of target expression and engagement, mechanism of action, and pharmacodynamic response.
Explore why modern clinical research requires advanced protein assays that go beyond analysis of protein presence and localization to enable quantification and functional interpretation of protein signals.
Sapient’s off-the-shelf protein assays already encompass more than 12,000 proteins. Any of these assays can undergo further qualification based on your specific study, sample type, and regulatory requirements, including with absolute quantification.
We can also develop bespoke assays for difficult-to-measure proteins outside of our existing menu, including for non-canonical proteins. In addition to endogenous proteins of interest, our workflows also enable targeted measurement of viral proteins, bacterial proteins, and drug levels.
Don’t see your protein of interest? Sapient offers custom protein assay services to develop bespoke & difficult-to-measure proteins, protein variants, and PTMs, including for multiplexed panels.
While traditional antibody-based protein assays are indirect, Sapient’s mass spectrometry-based workflows leverage labeled peptides for direct quantitation of proteins of interest.
We leverage next-generation mass spectrometry systems that significantly improve sensitivity, speed, and throughput to provide precise protein measurements at scale.
With the ability to process hundreds of samples per day, our targeted protein assay services are ideal for detecting and monitoring protein targets and biomarkers in large-scale population studies and clinical trials.
Unlike antibody-based detection approaches (e.g., IHC, ELISA, traditional ligand‑binding assays), Sapient’s targeted assays:
This makes them especially well‑suited for biomarker validation, target engagement, and translational-to-clinical studies where precision and reproducibility are critical. They can also be used as a complement to orthogonally validate findings obtained through traditional assay approaches and to further probe the functional biology behind targeted proteins.
Yes. Our targeted, quantitative protein assays can be multiplexed into customized panels, enabling the simultaneous measurement of multiple proteins within a single run. Panels can be designed based on biological pathways, disease areas, or study objectives to maximize data density while preserving quantitative rigor.
Sapient offers >12,000 off-the-shelf assays already measured via our mass spectrometry platform that can be rapidly optimized to your specific study requirements. Timelines are also significantly shorter for bespoke custom assay development compared to traditional immunoassay workflows since no antibody development time is required.
Sapient offers robust research use only (RUO) assays to support clinical biomarker confirmation/verification. We can provide analytical validation of targeted protein assays with appropriate context of use (COU) statements.
This ensures that the targeted assay data we deliver is not only clinically meaningful, but clinically actionable.
Sapient’s high throughput mass spectrometry systems allow us to deliver precision protein measures at scale, processing hundreds of samples per day and providing an ideal solution for detecting and monitoring protein targets and biomarkers in large-scale population studies and clinical trials.
For questions or to discuss a specific project, we encourage you to book a time to talk to our scientific team.
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